rat anti tubulin primary antibody  (Bio-Rad)

Journal: Journal of Experimental Botany

Article Title: Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES

doi: 10.1093/jxb/erac225

Figure Lengend Snippet: Spindle formation morphology in wild-type (WT, Col 0) and male sterile meiocytes. The spindle was detected by immunostaining with anti-α-tubulin antibody (green) and chromosomes were counterstained with DAPI (blue). Tubulin localization is similar in WT Col-0 and ams at prophase I (A, B), with normal spindle morphology during metaphase I in Col-0 (C) and ams (D). Radial microtubule arrays (RMA) however are disorganized in ams (F) compared with WT (E); this is also observed in the upstream male sterile mutants dyt1 (G) and tdf1 (H), but not the downstream mutant ms188 (I). Nucleus positioning within the tetrad is also unbalanced forming a ‘triad’ like shape in dyt1 , tdf1 , and ams (G–I). Scale bars: 5 μm.

Article Snippet: After rinsing with potassium phosphate buffer, immobilized cells were then incubated overnight at room temperature with rat α-tubulin primary antibody (0.3%; clone B-5-1-2; Sigma-Aldrich) in phosphate-buffered saline (PBS) containing 0.1% Triton X-100 and 4.5 g l −1 BSA.

Techniques: Sterility, Immunostaining, Mutagenesis